High pure viral nucleic acid kit roche manual

The High Pure Viral RNA Kit is for general laboratory use and purifies viral RNA from a variety of samples such as serum and plasma. Remove inhibitors that interfere with downstream assays, ensuring greater assay specificity, sensitivity, and reproducibility. Prepare RNA samples in only 20 minutes. The High Pure Viral Nucleic Acid Kit is intended for general laboratory use and efficiently purifies viral nucleic acids from serum, plasma, or whole blood using sample volumes of up to μL. When using whole blood as starting material, total nucleic acids are isolated, including viral nucleic acids. The MagNA Pure LC Total Nucleic Acid Isolation Kit - High Performance is designed to isolate high-purity total nucleic acid (e.g., viral DNA and RNA) from mammalian serum, plasma, or whole blood using MagNA Pure LC Instruments. The purified nucleic acid can be used directly in PCR on the LightCycler ® Carousel-Based Systems, the LightCycler ® System, or standard thermal block cyclers.

Methods: We therefore evaluated the LightCycler CMV Quant Kit (Roche Diagnostics) assay on whole blood after a manual extraction (High Pure viral nucleic acid kit, Roche Diagnostics), using as a reference an in-house Taqman assay (LC1UL83) which has been validated in various clinical situations. A panel obtained by serial dilutions of a virion. Choose the High Pure Viral Nucleic Acid Kit to iso-late viral DNA and RNA from serum, plasma, cell culture supernatant, or whole blood. The highly Consult this manual to see how Roche Applied Science's nucleic acid preparation products can help you optimize your nucleic acid isolation results. Nucleic acid isolation from these samples was performed by manual High Pure Viral Nucleic Acid Isolation kit (Roche Diagnostics, Mannheim, Germany), automated MagNA Pure LC Total Nucleic Acid Isolation kit (Roche Diagnostics), and automated Cobas AmpliPrep kit (Roche Diagnostics).

The MagNA Pure LC Total Nucleic Acid Isolation Kit - High Performance is designed to isolate high-purity total nucleic acid (e.g., viral DNA and RNA) from mammalian serum, plasma, or whole blood using MagNA Pure LC Instruments. The purified nucleic acid can be used directly in PCR on the LightCycler ® Carousel-Based Systems, the LightCycler ® System, or standard thermal block cyclers. High Pure PCR Template Preparation Kit; Instructions For Use Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants. Item High Pure Viral Nucleic Acid Kit; Company Roche; Price Get Quote Get Quote for this Product; Catalog Number ; Quantity 1 kit (for up to purifications) Source Blood, Plasma/Serum; Nucleic Acid Type Viral DNA, Viral RNA.